•Neutrophils
respond to infections and release extracellular traps (NETs), which are
antimicrobial and are made of DNA, histones, and neutrophil proteins. The
timely removal of NETs may be crucial for tissue homeostasis to avoid
presentation of self-antigens
•Excessive
NET formation is linked to several diseases
•Imaging
based phenotypic HTS of human primary neutrophils (190k compound library)
identified multiple sub µM hits series for different phenotypes
•Validation
of hit compounds through assay cascade and additional functional PBMC assays