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Anti-integrin αvβ6 antibody_Cancer, Fibrosis
CRUK
Antibody
Oncology
Target Identification or Validation
A series of anti-αvβ6 antibodies has been generated through a loop grafting approach, which involved taking advantage of the A20FMDV2 peptide, which has high affinity and selectivity for αvβ6 was inserted into CEA Antibody. Therefore, the series of anti-αvβ6 antibodies includes antibodies engineered to be highly αvβ6-specific and an antibody which also cross-reacts with the CEA. Importantly, B6.2 was determined to be as structurally stable as the parent scFv, indicating that insertion of the A20FMDV2 peptide and the Y100bP mutation (removes CEA cross-reactivity) was not detrimental to the protein structure. The humanised variant B6.3 has been generated in diabody and full IgG formats, the diabody variant of which has a Kd of 2.78 nM (as measured by Biacore).
US 8383593 B2